This was the ’90s, so my billowing clothing was on trend, but my skinny wrists were definitely not. Mediums and larges that billowed and ballooned over my small frame. So as I hit my first growth spurts I ambitiously bought clothing that I assumed I would grow into. I always held up hope that I was still, literally, on the rise. My dad is a robust 5’11”, and it seemed perfectly reasonable that I’d eclipse him someday. What did she know? From her perspective I kept getting bigger and bigger. My mom tried to comfort me by assuring me I’d grow up to be tall. But “little runt” was much harder to swallow. But I had seen a flash of bubble script, and after much pleading and prying, I got the book from him, flipped to my name, and saw the only words someone had cared to offer: “Annoying little runt.” The “annoying” didn’t bother me so much it was something I could control, and, as a middle school boy, being annoying was almost a point of pride. My buddy Ben, in a remarkably aware moment for a middle schooler, promptly snapped the book shut, claiming that the page was empty.
#SMEDIUM FOR MEDIUM FULL#
We flipped to the pages of my friends first, which were full of fawning praise - “So cute!”, “So funny!” - and then finally to mine. A couple friends and I had gotten our hands on the black book the girls had been passing around the hallways, which listed every boy in our grade, and, in colorful, anonymous bubble script that foreshadowed the future cruelty of the internet message board, opinions about each boy. Thus, the miniaturized cellular model system enables the recapitulation of a physiologically responsive, differentiated small airway epithelium, and a robotic integration provides a medium throughput approach towards pharmaceutical drug discovery, for instance, in respect of fibrotic distal airway/lung diseases.The most crushing moment of my middle school experience, a time when crushing moments seem to queue restlessly, occurred in health class. 96-well hSAE cultures were responsive to transforming growth factor $\beta$1 (TGF-$\beta$1) and tumor necrosis factor $\alpha$ (TNF-$\alpha$) in a concentration dependent manner. A novel custom-made device for 96-parallelized transepithelial electric resistance (TEER) measurements, together with dextran permeability measurements, confirmed that the 96-well culture developed a tight barrier function during ALI differentiation. The 96-well ALI cultures displayed a cellular composition, ciliary beating frequency, and intercellular tight junctions similar to 24-well conditions. After four weeks of ALI culture, a pseudostratified epithelium containing basal, club, goblet and ciliated cells was produced. Thus, a 96-well microplate system was developed where primary human small airway epithelial (hSAE) cells were cultured at an air-liquid interface (ALI). In order to overcome the challenges associated with a limited number of airway epithelial cells that can be obtained from clinical sampling and their restrained capacity to divide ex vivo, miniaturization of respiratory drug discovery assays is of pivotal importance.
Tissue and Cell Culture Dissociation Reagents.Work at STEMCELL View Current Opportunities >
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